One of the promising research directions for biopolymer-based applications is lab-on-chip as a microfluidic platform for cell culture purposes. Advantage of such a platform over the classical approach using a Petri dish is the possibility to emulate a mechanically compliant microfluidic environment in a well-controlled and reproducible manner. However, measurements of physical or biochemical parameters in close proximity to the cells (in situ) are desirable to characterize tissue functionality and cell responses, e.g., in the presence of drugs. Therefore, the integration of sensors into such platforms is essential for cell culture. Corresponding solutions for tailored approaches for tissue engineering and single cell experiments will be presented.